MCI GEL™ CHP series are suitable for reversed-phase chromatography and there are four kinds of columns of various hydrophobicity: Porous polystyrene, Modified porous polystyrene, Polymethacrylates, and Modified porous polymethacrylates. Thus, the proper kind of columns can be selected in accordance with the properties of the target compounds.
Polystyrene packing: MCI GEL™ CHP20/C04, CHP20/C10
Modified polystyrene packing: MCI GEL™ CHP07/C04, CHP07/C10, CHK40/C04
Polymethacrylates packing: MCI GEL™ CMG20/C10
Modified polymethacrylate packing: MCI GEL™ CHPOD/C04
The hydrophobicities of the columns are in the following orders: MCI GEL™ CHP07/C04 = CHP07/C10 > CHP20/C04 = CHP20/C10 > CHPOD/C04 ≥ ODS columns ≥ CMG20/C04 = CMG20/C10
Polymer columns for HPLC, with superior chemical resistance, can be applied with various mobile phases of broad range, acidic through alkaline. They have the following advantages due to their high hydrophobicity:
1. In reversed-phase distribution chromatography to separate acidic or alkaline compounds, the eluents suppressing ionic properties of such compounds are generally used. Polymer columns can be applied for the unsuitable compounds in ODS columns.
2. Some high hydrophilic compounds, e.g., amino acids, can be separated with strong hydrophobic CHP07/C04 and CHP07/C10 column.
3. Polymer columns can be washed with acidic and/or basic solutions when deteriorated by contamination.
Polymethacrylates, CMG20/C04 and CMG20/C10, can be applied not only for reversed-phase distribution chromatography but also for normal phase.
Modified polystyrene packing CHK40/C04 is a mix-mode type; both hydrophobic and hydrophilic interaction occur between packing material surface and analytes. It is useful for the compounds that are difficult to separate using existing ODS column or polymeric column. This column is also used in normal phase mode with unique separation profile.
All polymeric columns have superior stability and yield comparing ODS columns which have remaining silanol groups.
